Food and a primary link to human cancer: developing techniques to measure a potent carcinogen presen
Project Reference: 02-PG-027
Commencement Date: October, 2002
Project Duration: 48 months
One of the research recommendations that emerged from the World Health Organisation (WHO)/Food and Agriculture Organisation (FAO) Acrylamide in Food Network was the need to develop simple low-cost method(s) to be used for routine monitoring.
safefood sponsored a research project to design rapid and accurate tests for this carcinogenic compound. Two antibody based tests – ELISA and biosensor immunoassays – were produced and were shown to give rapid, quantitative and reproducible results for large numbers of food samples and across a wide range of food matrices.
In addition, these methods were shown to detect adducted acrylamide in human blood samples. Rapid sample preparation permits the analysis of a greater number of samples per day than would be possible with an equivalent LC/MS-MS system. Both ELISA and biosensor immunoassays for the detection of acrylamide in food were the first of their kind.
Prof Chris Elliott, Queen’s University Belfast
Laboratory capacity building
Preston, A., Fodey, T., Elliott, C.*.(2008).“Development of a high-throughput enzyme-linked immunosorbent assay for the routine detection of the Carcinogen Acylamide in food, via rapid derivation pre analysis”. Analytica Chimica acta 608 (2008) 178-185.
Preston, A*, Fodey, T., Douglas, A., Elliott, C. “Monoclonal antibody development for Acrylamide-adducted human haemoglobin; A biomarker of dietary acrylamide exposure”. Journal of Immunological Methods 341 (2009) 19-29.