Development of rapid tests for the detection of Mycobacterium paratuberculosis and their use to dete
Project Reference: 00-RESR-060
Status: Completed
Commencement Date: February, 2001
Project Duration: 36 months
Abstract:
This project was run in collaboration with Food Institutional Research Measure (FIRM), examined a number of aspects of MAP. The project involved a survey of faeces and milk from high-risk herds using culture-based and molecular detection methods.
The project also aimed to develop rapid methods for the detection of MAP in milk and other dairy products. The effect of different pasteurisation conditions on survival of MAP in milk was also under investigation.
Principal Contractor(s):
Ms Majella Maher, National Diagnostics Centre, Galway
Collaborator(s):
Dr Michael Rowe, Queen’s University Belfast
Outputs:
Reports:
Laboratory capacity-building
Peer Reviews:
Grant, I.R., Pope, C.M., O’Riordan, L.M., Ball, H.J. and Rowe, M.T. (2000). “Improved detection of Mycobacterium avium subsp. Paratuberculosis in milk by immunomagnetic PCR”. Veterinary Microbiology, 77(3-4):369-378.
Dundee, L., Grant, I.R, Ball, H.J. and Rowe, M.T. (2001). “Comparative evaluation of four decontamination protocols for the isolation of Mycobacterium avium subsp. Paratuberculosis from milk”. Letters in Applied Microbiology, 33:173-177.
Grant, I.R., O’Riordan, L.M., Ball, H.J and Rowe, M.T. (2001). “Incidence of Mycobacterium Paratuberculosis in raw sheep and goats’ milk in England, Wales and Northern Ireland”. Veterinary Microbiology, 79(2):123-131.
Grant, I.R.,Rowe, M.T., Dundee, L. and Hitchings, E.I.(2001). “Mycobacterium avium subsp. Paratuberculosis: its incidence, heat resistance and detection in milk and dairy products”. International Journal of Dairy Technology, 54(1):1-12.
Whan, L., Grant, I.R., Ball, H.J., Scott, R. and Rowe, M.T. (2001). “Bactericidal effect of chlorine on Mycobacterium Paratuberculosis in drinking water”. Letter in Applied Microbiology, 33:227-231.
Grant, I.R., Ball, H.J.,Rowe,M.T. (2002). “Incidence of Mycobacterium Paratuberculosis in bulk raw milk and commercially pasteurised cows’ milk from approved dairy processing establishments in the United Kingdom”. Applied and Environmental Microbiology, 68(5):2428-2435.
Grant, I.R., Hitchings, E.I., McCartney, A., Ferguson, F. and Rowe, M.T. (2002). “Effect of commercial-scale HTST pasteurisation on the viability of Mycobacterium paratuberculosis in naturally infected cows’ milk”. Applied and Environmental Microbiology, 68(2):602-607.
Donaghy, J.A., Totten, N.L. and Rowe, M.T. (2003). “Development of a laboratory-scale technique to monitor the persistence of Mycobacterium avium subsp. Paratuberculosis in cheddar cheese”. The Scientific Journal, 3:1241-1248.Donaghy, J.A., Totten, N.L. and Rowe, M.T. (2003). “Evaluation of culture media for the recovery of Mycobacterium avium subsp. Paratuberculosis from Cheddar cheese”. Letter in Applied Microbiology, 37:285-291.
Grant, I.R., Kirk, R.B., Hitchings, E.I., and Rowe, M.T. (2003). “Comparative evaluation of the MGIT and BACTEC culture systems for the recovery of Mycobacterium avium subsp. Paratuberculosis from milk”. Journal of Applied Microbiology, 95:196-201.
McKee, R., McCleery, D.R., Rowe, M.T., Murphy, B.P., Buckley, H.G., Buckley, J.F, Gilroy, D. and Fanning, S. (2003). “Examination of in-line milk filters to detect necrotoxigenic Escherichia coli at farm level”. Irish Journal of Agriculture and Food Research, 42(1):143-144.
Murphy, B.P., Fanning, S., Buckley, H.G., McKee, R., McCleery, D.R., Rowe, M.T. and Gilroy, D. (2003). “Pathogenic microorganisms in water samples from dairy farms in the Cork region”. Irish Journal of Agricultural and Food research, 42(1):163-164.
Donaghy, J.A., Totten, N.L. and Rowe, M.T. (2004). “Persistence of Mycobacterium Paratuberculosis during the manufacture and ripening of Cheddar cheese”. Applied and Environmental Microbiology, 70(8):4899-4905.
Grant, I.R. and Rowe, M.T. (2004). “Effect of chemical decontamination and refrigerated storage on the isolation of mycobacterium avium subsp. Paratuberculosis from heat-treated milk”. Letters in Applied Microbiology, 38(4): 283-288.
O’Reilly, C.E., O’Connor, L., Anderson, W., Harvey, P., Grant, I.R., Donaghy, J.A, Rowe, M.T. and O’Mahony, P. (2004). “Surveillance of bulk raw and commercially pasteurized cows’ milk from approved Irish Liquid-milk pasteurization plants to determine the incidence of Mycobacterium Paratuberculosis”. Applied and Environmental Microbiology, 71(9):5138-5144.
Grant, I.R. Williams, A.G., Rowe, M.T. and Muir, D.D. (2005). “Efficacy of various pasteurisation time/temperature conditions in combination with homogenisation on the inactivation of mycobacterium avium subsp. Paratuberculosis in milk”. Applied and Environmental Microbiology, 71(6): 2853-2861.
Grant, I.R. Williams, A.G., Rowe, M.T. and Muir, D.D. (2005). “Investigation of the impact of simulated commercial centrifugation and microfiltration conditions on levels of mycobacterium avium subsp. Paratuberculosis in milk”. International Journal of Dairy Technology, 58(3): 138-142.
Murphy, B.P., Murphy, M., Buckley, J.F., Gilroy, D., Rowe, M.T., McCleery, D.R. and Fanning, S. (2005). “In-line milk filters analysis: Escherichia coli O157 surveillance of milk production holdings”. International Journal of Hygiene and Environmental health, 208(5):407-413.
Whan, L., Ball, H.J., Grant, I.R. and Rowe, M.T. (2005). “Development of an IMS-PCR assay for the detection of Mycobacterium avium subsp. Paratuberculosis in water”. Letters in Applied Microbiology, 40:269-273.
Whan, L., Ball, H.J., Grant, I.R. and Rowe, M.T. (2005). “Occurrence of Mycobacterium avium subsp. Paratuberculosis in raw waters in Northern Ireland”. Applied and Environmental Microbiology, 71:7107-7112.
Rowe, M.T. and Grant, I.R. (2006). “Mycobacterium avium subsp. Paratuberculosis and its potential survival tactics”. Letters in Applied Microbiology, 42:305-311.
Whan, L., Grant, I.R. and Rowe, M.T. (2006). “Interaction between Mycobacterium avium subsp. Paratuberculosis and environmental protozoa”. BMC Microbiology,6:1-6.
Altic, L.C., Rowe, M.T. and Grant, I.R. (2007). “UV light inactivation of Mycobacterium avium subsp. Paratuberculosis in milk assessed by Fast Plaque assay and culture”. Applied and Environmental Microbiology, 73(11):3728-3733.
Donaghy, J.A., Linton, M., Patterson, M.F and Rowe, M.T. (2007). “Effect of high pressure and pasteurisation on Mycobacterium avium ssp. Paratuberculosis in milk”. Letters in Applied Microbiology, 45(2):154-159.
Donaghy, J.A., Rowe, M.T., Rademaker, J., Hammer, P., Herman, L. De Jonghe, V., Blanchard, B., Duhem, K. and Vindel, E. (2008). “An inter-laboratory trial for the detection and isolation of Mycobacterium avium subsp. Paratuberculosis from raw milk artificaially contacminated with naturally infected faeces”. Food Microbiology, 25:128-135.
Donaghy, J.A.; Keyser, M.; Johnston, Jane, Cilliers T.; Gouws, P. and Rowe, M.T. (2009). “Inactivation of Mycobacterium avium ssp. Paratuberculosis in milk by UV treatment”. Letters in Applied Microbiology, 49:217-221.
Aboagye, G. and Rowe, M.T. (2011). “Occurrence of Mycobacterium avium subsp. Paratuberculosis in raw water and water treatment operations for the production of potable water”. Water Research, 45:3271-3278.
Donaghy, J.A., Johnston, Jane and Rowe, M.T.(2011). “Detection of Mycobacterium avium ssp. Paratuberculosis in cheese, milk powder and milk using IS900 and f-57 based qPCR”. Journal of Applied Microbiology. 110(2):479-489.
